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KMID : 0981820070270060458
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Korean Journal of Laboratory Medicine
2007 Volume.27 No. 6 p.458 ~ p.463
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Development of a Web-based Program for the Identification of Human Leukocyte Antigen Antibody Specificities
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Cha Choong-Hwan
Oh Heung-Bum
Kim Myeong-Hee
Chae Jeong-Min
Jung Soon-Young
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Abstract
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Background: Panel reactive antibody (PRA) test is used to determine whether a patient awaiting transplantation is previously sensitized. Tail analysis algorithm is widely used to identify antibody specificities, but it is very difficult to perform manually.
Methods: To develop a web-based program, PHP (5.1.2), Apache (2.0.55), and MySQL (5.0.22) were used. Tail analysis algorithm was applied to identify specificities, which analyzed statistically 2×2 tables representing reactivities to broad antigens, splits and cross reactive groups (CREG). Exploiting two CREG classifications of Rodey (R) and Takemoto (T), antibody specificities were identified by 3 methods (ABC, R-ABC, T-ABC) simultaneously. Performance of the system was evaluated using 159 samples that showed ≥6 PRA% by a lymphocytotoxicity assay.
Results: A web-based system that can identify HLA antibody specificities was implemented on www.koreanhla.com. Among 159 samples tested, antibody specificities were identified in 151 (95.0 %), but not in 8 samples with PRA >97%. Among the 151 samples, 110 showed broad or split specificities and 41 CREG specificities.
Conclusion: We developed a web-based computer program for the identification of HLA antibody specificities. Accessible to everyone on the internet, this program should be of help in sharing PRA results among laboratories. (Korean J Lab Med 2007;27:458-63)
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KEYWORD
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Panel reactive antibody (PRA), Tail analysis, Human leukocyte antigens (HLA)
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